Bcl10 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54557
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54557-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54557-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the BCL10 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human tonsil tissue with BCL10 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of human HeLa cells with BCL10 antibody (green) and DAPI nuclear stain (blue).
3 / 4
Western blot testing of mouse spleen lysate with BCL10 antibody. Observed molecular weight: 26~33 kDa.
4 / 4
Flow cytometry testing of human MCF7 cells with BCL10 antibody; Blue=isotype control, Green= BCL10 antibody.
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IHC testing of FFPE human tonsil tissue with BCL10 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HeLa cells with BCL10 antibody (green) and DAPI nuclear stain (blue).
Western blot testing of mouse spleen lysate with BCL10 antibody. Observed molecular weight: 26~33 kDa.
Flow cytometry testing of human MCF7 cells with BCL10 antibody; Blue=isotype control, Green= BCL10 antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25, Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the BCL10 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 24-51 from the human protein was used as the immunogen for the BCL10 antibody.
Limitation:
This BCL10 antibody is available for research use only.
Localization:
Cytoplasmic, perinuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
O95999