Endothelin B Receptor Antibody / EDNRB

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NSJ Bioreagents
Product Code: NSJ-F54884
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54884-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54884-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Endothelin B Receptor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human prostate carcinoma tissue with Endothelin B Receptor antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of human HEK292 cells with Endothelin B Receptor antibody (green) and DAPI nuclear stain (blue).
3 / 4
Western blot testing of mouse cerebellum tissue lysate with Endothelin B Receptor antibody. Predicted molecular weight ~50 kDa.
4 / 4
Flow cytometry testing of human MDA-MB-435 cells with Endothelin B Receptor antibody; Blue=isotype control, Green= Endothelin B Receptor antibody.
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IHC testing of FFPE human prostate carcinoma tissue with Endothelin B Receptor antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HEK292 cells with Endothelin B Receptor antibody (green) and DAPI nuclear stain (blue).
Western blot testing of mouse cerebellum tissue lysate with Endothelin B Receptor antibody. Predicted molecular weight ~50 kDa.
Flow cytometry testing of human MDA-MB-435 cells with Endothelin B Receptor antibody; Blue=isotype control, Green= Endothelin B Receptor antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE): 1:10-1:50, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Endothelin B Receptor antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
EDNRB is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 409-439 from the human protein was used as the immunogen for the Endothelin B Receptor antibody.
Limitation:
This Endothelin B Receptor antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
P24530