PSMB1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54465
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54465-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54465-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the PSMB1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of human 1) K562 and 2) MCF7 cell lysate with PSMB1 antibody. Predicted molecular weight ~26 kDa.
2 / 5
Western blot testing of mouse 1) NIH 3T3 and 2) bladder lysate with PSMB1 antibody. Predicted molecular weight ~26 kDa.
3 / 5
Immunofluorescent staining of human HepG2 cells with PSMB1 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 5
IHC testing of FFPE human brain tissue with PSMB1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 5
Flow cytometry testing of human HL60 cells with PSMB1 antibody; Blue=isotype control, Green= PSMB1 antibody.
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Western blot testing of human 1) K562 and 2) MCF7 cell lysate with PSMB1 antibody. Predicted molecular weight ~26 kDa.
Western blot testing of mouse 1) NIH 3T3 and 2) bladder lysate with PSMB1 antibody. Predicted molecular weight ~26 kDa.
Immunofluorescent staining of human HepG2 cells with PSMB1 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
IHC testing of FFPE human brain tissue with PSMB1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HL60 cells with PSMB1 antibody; Blue=isotype control, Green= PSMB1 antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells),Immunofluorescence: 1:25,Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the PSMB1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PSMB1 is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 214-241 from the human protein was used as the immunogen for the PSMB1 antibody.
Limitation:
This PSMB1 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P20618