Cofilin 1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54403
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54403-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54403-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
Aliquot the CFL1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Flow cytometry testing of human HL60 cells with CFL1 antibody; Blue=isotype control, Green= CFL1 antibody.
2 / 5
Western blot testing of human MCF7 cell lysate with CFL1 antibody. Predicted molecular weight ~19 kDa.
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Western blot testing of human HL60 cell lysate with CFL1 antibody. Predicted molecular weight ~19 kDa.
4 / 5
Western blot testing of mouse NIH 3T3 cell lysate with CFL1 antibody. Predicted molecular weight ~19 kDa.
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Western blot testing of 1) non-transfected and 2) transfected HEK293 cell lysate with CFL1 antibody.
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Flow cytometry testing of human HL60 cells with CFL1 antibody; Blue=isotype control, Green= CFL1 antibody.
Western blot testing of human MCF7 cell lysate with CFL1 antibody. Predicted molecular weight ~19 kDa.
Western blot testing of human HL60 cell lysate with CFL1 antibody. Predicted molecular weight ~19 kDa.
Western blot testing of mouse NIH 3T3 cell lysate with CFL1 antibody. Predicted molecular weight ~19 kDa.
Western blot testing of 1) non-transfected and 2) transfected HEK293 cell lysate with CFL1 antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells),Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the CFL1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 70-98 from the human protein was used as the immunogen for the CFL1 antibody.
Limitation:
This CFL1 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P23528