PAICS Antibody / ADE2

NSJ Bioreagents
Product Code: NSJ-F54659
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54659-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54659-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the ADE2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human colon carcinoma tissue with ADE2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of mouse NIH 3T3 cell lysate with ADE2 antibody. Predicted molecular weight ~47 kDa.
3 / 4
Western blot testing of human K562 cell lysate with ADE2 antibody. Predicted molecular weight ~47 kDa.
4 / 4
Flow cytometry testing of human K562 cells with ADE2 antibody; Blue=isotype control, Green= ADE2 antibody.

IHC testing of FFPE human colon carcinoma tissue with ADE2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of mouse NIH 3T3 cell lysate with ADE2 antibody. Predicted molecular weight ~47 kDa.
Western blot testing of human K562 cell lysate with ADE2 antibody. Predicted molecular weight ~47 kDa.
Flow cytometry testing of human K562 cells with ADE2 antibody; Blue=isotype control, Green= ADE2 antibody.

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the ADE2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The PAICS gene encodes a bifunctional enzyme containing phosphoribosylaminoimidazole carboxylase activity in its N-terminal region and phosphoribosylaminoimidazole succinocarboxamide synthetase in its C-terminal region. It catalyzes steps 6 and 7 of purine biosynthesis. The gene is closely linked and divergently transcribed with a locus that encodes an enzyme in the same pathway, and transcription of the two genes is coordinately regulated. The human genome contains several pseudogenes of this gene. Multiple transcript variants encoding different isoforms have been found for this gene.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 95-123 from the human protein was used as the immunogen for the ADE2 antibody.
Limitation:
This ADE2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P22234