Alcohol dehydrogenase 4 Antibody / ADH4

NSJ Bioreagents
Product Code: NSJ-F54534
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54534-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54534-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Alcohol dehydrogenase 4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Alcohol dehydrogenase 4 antibody. Predicted molecular weight ~40 kDa.
2 / 4
Western blot testing of mouse heart tissue with Alcohol dehydrogenase 4 antibody. Predicted molecular weight ~40 kDa.
3 / 4
IHC testing of FFPE human colon carcinoma tissue with Alcohol dehydrogenase 4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human HEK293 cells with Alcohol dehydrogenase 4 antibody; Blue=isotype control, Green= Alcohol dehydrogenase 4 antibody.

Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Alcohol dehydrogenase 4 antibody. Predicted molecular weight ~40 kDa.
Western blot testing of mouse heart tissue with Alcohol dehydrogenase 4 antibody. Predicted molecular weight ~40 kDa.
IHC testing of FFPE human colon carcinoma tissue with Alcohol dehydrogenase 4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HEK293 cells with Alcohol dehydrogenase 4 antibody; Blue=isotype control, Green= Alcohol dehydrogenase 4 antibody.

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Alcohol dehydrogenase 4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 319-348 from the human protein was used as the immunogen for the Alcohol dehydrogenase 4 antibody.
Limitation:
This Alcohol dehydrogenase 4 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P08319