BLOC1S2 Antibody / BLOC-1 subunit 2

NSJ Bioreagents
Product Code: NSJ-F54828
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54828-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54828-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the BLOC1S2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of mouse NIH 3T3 cell lysate with BLOC1S2 antibody. Predicted molecular weight ~16 kDa.
2 / 4
Western blot testing of human HepG2 cell lysate with BLOC1S2 antibody. Predicted molecular weight ~16 kDa.
3 / 4
IHC testing of FFPE human cervical carcinoma tissue with BLOC1S2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human HepG2 cells with BLOC1S2 antibody; Blue=isotype control, Green= BLOC1S2 antibody.

Western blot testing of mouse NIH 3T3 cell lysate with BLOC1S2 antibody. Predicted molecular weight ~16 kDa.
Western blot testing of human HepG2 cell lysate with BLOC1S2 antibody. Predicted molecular weight ~16 kDa.
IHC testing of FFPE human cervical carcinoma tissue with BLOC1S2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HepG2 cells with BLOC1S2 antibody; Blue=isotype control, Green= BLOC1S2 antibody.

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the BLOC1S2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
BLOC1S2 is a component of the ubiquitously expressed BLOC1 multisubunit protein complex. BLOC1 is required for normal biogenesis of specialized organelles of the endosomal-lysosomal system, such as melanosomes and platelet dense granules (Starcevic and Dell'Angelica, 2004 [PubMed 15102850]).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 14-40 from the human protein was used as the immunogen for the BLOC1S2 antibody.
Limitation:
This BLOC1S2 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q6QNY1