Calcipressin-2 Antibody / RCAN2 / DSCR1L1

NSJ Bioreagents
Product Code: NSJ-F54849
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54849-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54849-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Calcipressin-2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of mouse heart tissue lysate with Calcipressin-2 antibody. Predicted molecular weight ~22 kDa.
2 / 4
Western blot testing of NCI-H460 cell lysate with Calcipressin-2 antibody. Predicted molecular weight ~22 kDa.
3 / 4
IHC testing of FFPE human brain tissue with Calcipressin-2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human NCI-H460 cells with Calcipressin-2 antibody; Blue=isotype control, Green= Calcipressin-2 antibody.

Western blot testing of mouse heart tissue lysate with Calcipressin-2 antibody. Predicted molecular weight ~22 kDa.
Western blot testing of NCI-H460 cell lysate with Calcipressin-2 antibody. Predicted molecular weight ~22 kDa.
IHC testing of FFPE human brain tissue with Calcipressin-2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human NCI-H460 cells with Calcipressin-2 antibody; Blue=isotype control, Green= Calcipressin-2 antibody.

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:10-1:50, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Calcipressin-2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Calcipressin-2/DSCR1L1 inhibits calcineurin-dependent transcriptional responses by binding to the catalytic domain of calcineurin A. This protein may play a role during central nervous system development. Expression is detected in fibroblasts, heart, brain, liver, and skeletal muscle but not in placenta, lung, kidney and pancreas. Expression of both transcripts is upregulated by physiologic concentrations of the thyroid hormone triiodothyroxine.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 6-37 from the human protein was used as the immunogen for the Calcipressin-2 antibody.
Limitation:
This Calcipressin-2 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q14206