FBXW8 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54559
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54559-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54559-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the FBXW8 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of mouse bladder tissue lysate with FBXW8 antibody. Predicted molecular weight ~67 kDa.
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Western blot testing of human HeLa cell lysate with FBXW8 antibody. Predicted molecular weight ~67 kDa.
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IHC testing of FFPE human lung carcinoma tissue with FBXW8 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Flow cytometry testing of human HeLa cells with FBXW8 antibody; Blue=isotype control, Green= FBXW8 antibody.
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Western blot testing of mouse bladder tissue lysate with FBXW8 antibody. Predicted molecular weight ~67 kDa.
Western blot testing of human HeLa cell lysate with FBXW8 antibody. Predicted molecular weight ~67 kDa.
IHC testing of FFPE human lung carcinoma tissue with FBXW8 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HeLa cells with FBXW8 antibody; Blue=isotype control, Green= FBXW8 antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the FBXW8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene encodes a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene contains a WD-40 domain, in addition to an F-box motif, so it belongs to the Fbw class. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 270-299 from the human protein was used as the immunogen for the FBXW8 antibody.
Limitation:
This FBXW8 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q8N3Y1