MAP3K15 Antibody

NSJ Bioreagents
Product Code: NSJ-F54647
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54647-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54647-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the MAP3K15 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human fetal brain lysate with MAP3K15 antibody. Predicted molecular weight ~147 kDa.
2 / 4
Western blot testing of mouse Neuro-2a cell lysate with MAP3K15 antibody. Predicted molecular weight ~147 kDa.
3 / 4
IHC testing of FFPE human kidney tissue with MAP3K15 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of mouse Neuro-2a cells with MAP3K15 antibody; Blue=isotype control, Green= MAP3K15 antibody.

Western blot testing of human fetal brain lysate with MAP3K15 antibody. Predicted molecular weight ~147 kDa.
Western blot testing of mouse Neuro-2a cell lysate with MAP3K15 antibody. Predicted molecular weight ~147 kDa.
IHC testing of FFPE human kidney tissue with MAP3K15 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of mouse Neuro-2a cells with MAP3K15 antibody; Blue=isotype control, Green= MAP3K15 antibody.

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the MAP3K15 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene is a member of the mitogen-activated protein kinase (MAPK) family. These family members function in a protein kinase signal transduction cascade, where an activated MAPK kinase kinase (MAP3K) phosphorylates and activates a specific MAPK kinase (MAP2K), which then activates a specific MAPK. This MAP3K protein plays an essential role in apoptotic cell death triggered by cellular stresses. [provided by RefSeq].
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 315-342 from the human protein was used as the immunogen for the MAP3K15 antibody.
Limitation:
This MAP3K15 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q6ZN16