PEA3 Antibody / ETV4

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NSJ Bioreagents
Product Code: NSJ-F54869
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54869-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54869-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the PEA3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with PEA3 antibody.
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Western bloot testing of mouse heart tissue lysate with PEA3 antibody. Expected molecular weight: 54-61 kDa.
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IHC testing of FFPE human brain tissue with PEA3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Flow cytometry testing of human WiDr cells with PEA3 antibody; Blue=isotype control, Green= PEA3 antibody.
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Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with PEA3 antibody.
Western bloot testing of mouse heart tissue lysate with PEA3 antibody. Expected molecular weight: 54-61 kDa.
IHC testing of FFPE human brain tissue with PEA3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human WiDr cells with PEA3 antibody; Blue=isotype control, Green= PEA3 antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:10-1:50, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the PEA3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ETV4/PEA3 is a transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 419-447 from the human protein was used as the immunogen for the PEA3 antibody.
Limitation:
This PEA3 antibody is available for research use only.
Localization:
Nuclear
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
P43268