PRP19 Antibody / Pre-mRNA-processing factor 19 / PRPF19

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NSJ Bioreagents
Product Code: NSJ-F54809
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54809-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54809-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the PRP19 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human lung carcinoma tissue with PRP19 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of human MCF7 cell lysate with PRP19 antibody. Predicted molecular weight ~55 kDa.
3 / 4
Western blot testing of mouse brain tissue lysate with PRP19 antibody. Predicted molecular weight ~55 kDa.
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Flow cytometry testing of human MCF7 cells with PRP19 antibody; Blue=isotype control, Green= PRP19 antibody.
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IHC testing of FFPE human lung carcinoma tissue with PRP19 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human MCF7 cell lysate with PRP19 antibody. Predicted molecular weight ~55 kDa.
Western blot testing of mouse brain tissue lysate with PRP19 antibody. Predicted molecular weight ~55 kDa.
Flow cytometry testing of human MCF7 cells with PRP19 antibody; Blue=isotype control, Green= PRP19 antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the PRP19 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PRPF19 is the human homolog of yeast Pso4, a gene essential for cell survival and DNA repair.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 4-33 from the human protein was used as the immunogen for the PRP19 antibody.
Limitation:
This PRP19 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q9UMS4