Twinfilin 2 Antibody / TWF2 / PTK9L

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NSJ Bioreagents
Product Code: NSJ-F54606
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54606-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54606-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Twinfilin 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of mouse lung lysate with Twinfilin 2 antibody. Predicted molecular weight ~39 kDa.
2 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Twinfilin 2 antibody. Predicted molecular weight ~39 kDa.
3 / 4
IHC testing of FFPE human tonsil tissue with Twinfilin 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human HEK293 cells with Twinfilin 2 antibody; Blue=isotype control, Green= Twinfilin 2 antibody.
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Western blot testing of mouse lung lysate with Twinfilin 2 antibody. Predicted molecular weight ~39 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Twinfilin 2 antibody. Predicted molecular weight ~39 kDa.
IHC testing of FFPE human tonsil tissue with Twinfilin 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HEK293 cells with Twinfilin 2 antibody; Blue=isotype control, Green= Twinfilin 2 antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Twinfilin 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene was identified by its interaction with the catalytic domain of protein kinase C-zeta. The encoded protein contains an actin-binding site and an ATP-binding site. It is most closely related to twinfilin (PTK9), a conserved actin monomer-binding protein.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 28-57 from the human protein was used as the immunogen for the Twinfilin 2 antibody.
Limitation:
This Twinfilin 2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q6IBS0