DOPA Decarboxylase Antibody / AADC / DDC

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NSJ Bioreagents
Product Code: NSJ-F54418
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54418-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54418-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the AADC antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of human kidney lysate with AADC antibody. Predicted molecular weight ~54 kDa.
2 / 5
Western blot testing of mouse 1) liver and 2) kidney lysate with AADC antibody. Predicted molecular weight ~54 kDa.
3 / 5
Immunofluorescent staining of human HepG2 cells with AADC antibody (green) and DAPI nuclear stain (blue).
4 / 5
IHC testing of FFPE human hepatocarcinoma tissue with AADC antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 5
Flow cytometry testing of human HepG2 cells with AADC antibody; Blue=isotype control, Green= AADC antibody.
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Western blot testing of human kidney lysate with AADC antibody. Predicted molecular weight ~54 kDa.
Western blot testing of mouse 1) liver and 2) kidney lysate with AADC antibody. Predicted molecular weight ~54 kDa.
Immunofluorescent staining of human HepG2 cells with AADC antibody (green) and DAPI nuclear stain (blue).
IHC testing of FFPE human hepatocarcinoma tissue with AADC antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HepG2 cells with AADC antibody; Blue=isotype control, Green= AADC antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000,Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the AADCantibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This protein catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Defects in this gene are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 32-61 from the human protein was used as the immunogen for the AADC antibody.
Limitation:
This AADC antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P20711