HSP90B1 Antibody / GRP94

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NSJ Bioreagents
Product Code: NSJ-F54810
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54810-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54810-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the HSP90B1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Flow cytometry testing of human K562 cells with HSP90B1 antibody; Blue=isotype control, Green= HSP90B1 antibody.
2 / 4
IHC testing of FFPE human lung carcinoma tissue with HSP90B1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of human NCI-H460 cells with HSP90B1 antibody (green) and DAPI nuclear stain (blue).
4 / 4
Western blot testing of 1) human HepG2, 2) mouse liver, 3) hamster CHO, 4) human K562 and 5) human HeLa cell lysate with HSP90B1 antibody. Predicted molecular weight ~94 kDa.
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Flow cytometry testing of human K562 cells with HSP90B1 antibody; Blue=isotype control, Green= HSP90B1 antibody.
IHC testing of FFPE human lung carcinoma tissue with HSP90B1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human NCI-H460 cells with HSP90B1 antibody (green) and DAPI nuclear stain (blue).
Western blot testing of 1) human HepG2, 2) mouse liver, 3) hamster CHO, 4) human K562 and 5) human HeLa cell lysate with HSP90B1 antibody. Predicted molecular weight ~94 kDa.

Documents

Further Information

Application Details :
Immunofluorescence: 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the HSP90B1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
HSP90B1 is highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is an endoplasmic reticulum HSP90 protein. Other HSP90 proteins are found in cytosol.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 16-43 from the human protein was used as the immunogen for the HSP90B1 antibody.
Limitation:
This HSP90B1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P14625