Engrailed 1 Antibody / EN1

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NSJ Bioreagents
Product Code: NSJ-F54331
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54331-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54331-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the Engrailed 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human 1) U-87 MG, 2) U-2 OS, 3) SH-SY5Y, 4) 293T, 5) HepG2 and 6) mouse brain lysate with Engrailed 1 antibody. Predicted molecular weight ~40 kDa.
2 / 3
Immunofluorescent staining of fixed and permeabilized human U-2 OS cells with Engrailed 1 antibody (green) and anti-Actin (red).
3 / 3
Immunofluorescent staining of fixed and permeabilized human U-251 cells with Engrailed 1 antibody (green) and anti-Actin (red).
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Western blot testing of human 1) U-87 MG, 2) U-2 OS, 3) SH-SY5Y, 4) 293T, 5) HepG2 and 6) mouse brain lysate with Engrailed 1 antibody. Predicted molecular weight ~40 kDa.
Immunofluorescent staining of fixed and permeabilized human U-2 OS cells with Engrailed 1 antibody (green) and anti-Actin (red).
Immunofluorescent staining of fixed and permeabilized human U-251 cells with Engrailed 1 antibody (green) and anti-Actin (red).

Documents

Further Information

Application Details :
Immunofluorescence: 1:25,Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Engrailed 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1-30 from the human protein was used as the immunogen for the Engrailed 1 antibody.
Limitation:
This Engrailed 1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q05925