CBS Antibody / Cystathionine Beta Synthase

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NSJ Bioreagents
Product Code: NSJ-F54436
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54436-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54436-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Cystathionine Beta Synthase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of 1) mouse kidney and 2) human Ramos lysate with Cystathionine Beta Synthase antibody. Predicted molecular weight ~61 kDa.
2 / 5
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Cystathionine Beta Synthase antibody.
3 / 5
Immunofluorescent staining of human HEK293 cells with Cystathionine Beta Synthase antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 5
IHC testing of FFPE human hepatocarcinoma tissue with Cystathionine Beta Synthase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 5
Flow cytometry testing of human Ramos cells with Cystathionine Beta Synthase antibody; Blue=isotype control, Green= Cystathionine Beta Synthase antibody.
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Western blot testing of 1) mouse kidney and 2) human Ramos lysate with Cystathionine Beta Synthase antibody. Predicted molecular weight ~61 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Cystathionine Beta Synthase antibody.
Immunofluorescent staining of human HEK293 cells with Cystathionine Beta Synthase antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
IHC testing of FFPE human hepatocarcinoma tissue with Cystathionine Beta Synthase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human Ramos cells with Cystathionine Beta Synthase antibody; Blue=isotype control, Green= Cystathionine Beta Synthase antibody.

Documents

Further Information

Application Details :
Immunofluorescence: 1:25,Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the Cystathionine Beta Synthase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CBS acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. This protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 104-133 from the human protein was used as the immunogen for the Cystathionine Beta Synthase antibody.
Limitation:
This Cystathionine Beta Synthase antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P35520