ATG7 Antibody / APG7

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NSJ Bioreagents
Product Code: NSJ-F54380
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54380-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54380-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the ATG7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
IHC testing of FFPE human liver tissue with ATG7 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
2 / 5
Western blot testing of 1) human HepG2 and 2) mouse NIH 3T3 cell lysate with ATG7 antibody. Predicted molecular weight: 70-80 kDa.
3 / 5
Western blot testing of human 1) Caki-1, 2) HeLa and 3) HepG2 cell lysate with ATG7 antibody. Predicted molecular weight: 70-80 kDa.
4 / 5
Western blot testing of human HEK293 cell lysate with ATG7 antibody. Predicted molecular weight: 70-80 kDa.
5 / 5
Immunofluorescent staining of treated and untreated human U-251 cells with ATG7 antibody (green) and DAPI nuclear stain (blue).
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IHC testing of FFPE human liver tissue with ATG7 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
Western blot testing of 1) human HepG2 and 2) mouse NIH 3T3 cell lysate with ATG7 antibody. Predicted molecular weight: 70-80 kDa.
Western blot testing of human 1) Caki-1, 2) HeLa and 3) HepG2 cell lysate with ATG7 antibody. Predicted molecular weight: 70-80 kDa.
Western blot testing of human HEK293 cell lysate with ATG7 antibody. Predicted molecular weight: 70-80 kDa.
Immunofluorescent staining of treated and untreated human U-251 cells with ATG7 antibody (green) and DAPI nuclear stain (blue).

Documents

Further Information

Application Details :
Immunofluorescence: 1:25,Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the ATG7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 540-569 from the human protein was used as the immunogen for the ATG7 antibody.
Limitation:
This ATG7 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
O95352