p21 Antibody / CDKN1A

Contact us
NSJ Bioreagents
Product Code: NSJ-F54392
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54392-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54392-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the CDKN1A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Western blot testing of human 1) HeLa, 2) HUVEC, 3) MCF7, 4) SH-SY5Y and 5) mouse lung lysate with CDKN1A antibody.
2 / 3
Immunofluorescent staining of fixed and permeabilized human A549 cells with CDKN1A antibody (green) and anti-Actin (red).
3 / 3
IHC testing of FFPE human colon tissue with CDKN1A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
❮❯

Western blot testing of human 1) HeLa, 2) HUVEC, 3) MCF7, 4) SH-SY5Y and 5) mouse lung lysate with CDKN1A antibody.
Immunofluorescent staining of fixed and permeabilized human A549 cells with CDKN1A antibody (green) and anti-Actin (red).
IHC testing of FFPE human colon tissue with CDKN1A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Immunofluorescence: 1:25,Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the CDKN1A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin- dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D- CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 133-164 from the human protein was used as the immunogen for the CDKN1A antibody.
Limitation:
This CDKN1A antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P38936