MEK2 Antibody / MAP2K2

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NSJ Bioreagents
Product Code: NSJ-F54450
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54450-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54450-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the MEK2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human U937 cell lysate with MEK2 antibody. Expected molecular weight: 45-50 kDa.
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Western blot testing of mouse liver lysate with MEK2 antibody. Expected molecular weight: 45-50 kDa.
3 / 5
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with MEK2 antibody.
4 / 5
Immunofluorescent staining of fixed and permeabilized human U-251 cells with MEK2 antibody (green) and anti-Actin (red).
5 / 5
IHC testing of FFPE human cancer tissue with MEK2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human U937 cell lysate with MEK2 antibody. Expected molecular weight: 45-50 kDa.
Western blot testing of mouse liver lysate with MEK2 antibody. Expected molecular weight: 45-50 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with MEK2 antibody.
Immunofluorescent staining of fixed and permeabilized human U-251 cells with MEK2 antibody (green) and anti-Actin (red).
IHC testing of FFPE human cancer tissue with MEK2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Immunofluorescence: 1:25,Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the MEK2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
MAP2K2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 262-292 from the human protein was used as the immunogen for the MEK2 antibody.
Limitation:
This MEK2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P36507