Tec Antibody

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NSJ Bioreagents
Product Code: NSJ-F54447
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54447-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54447-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the TEC antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human NCI-H292 cell lysate with TEC antibody. Expected molecular weight: 58-73 kDa (multiple isoforms).
2 / 4
Western blot testing of mouse liver lysate with TEC antibody. Expected molecular weight: 58-73 kDa (multiple isoforms).
3 / 4
Immunofluorescent staining of fixed and permeabilized human HeLa cells with TEC antibody (green) and anti-Actin (red).
4 / 4
IHC testing of FFPE human breast cancer tissue with Tec antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human NCI-H292 cell lysate with TEC antibody. Expected molecular weight: 58-73 kDa (multiple isoforms).
Western blot testing of mouse liver lysate with TEC antibody. Expected molecular weight: 58-73 kDa (multiple isoforms).
Immunofluorescent staining of fixed and permeabilized human HeLa cells with TEC antibody (green) and anti-Actin (red).
IHC testing of FFPE human breast cancer tissue with Tec antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Immunofluorescence: 1:25,Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the TEC antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
TEC belongs to the TEC subfamily of non-receptor protein-tyrosine kinases containing a pleckstrin homology domain. TEC family kinases are involved in the intracellular signaling mechanisms of cytokine receptors, lymphocyte surface antigens, heterotrimeric G-protein coupled receptors, and integrin molecules. They are also key players in the regulation of the immune functions. TEC kinase is an integral component of T cell signaling and has a distinct role in T cell activation. TEC may be associated with myelodysplastic syndrome.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 175-205 from the human protein was used as the immunogen for the TEC antibody.
Limitation:
This TEC antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P42680