Presenilin 1 Antibody / PSEN1

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NSJ Bioreagents
Product Code: NSJ-F54846
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54846-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54846-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Presenilin 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human 1) HeLa and 2) HL60 cell lysate with Presenilin 1 antibody. Predicted molecular weight ~53 kDa.
2 / 4
Immunofluorescent staining of human MDA-MB-435 cells with Presenilin 1 antibody (green) and DAPI nuclear stain (blue).
3 / 4
IHC testing of FFPE human cancer tissue with Presenilin 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human HeLa cells with Presenilin 1 antibody; Blue=isotype control, Green= Presenilin 1 antibody.
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Western blot testing of human 1) HeLa and 2) HL60 cell lysate with Presenilin 1 antibody. Predicted molecular weight ~53 kDa.
Immunofluorescent staining of human MDA-MB-435 cells with Presenilin 1 antibody (green) and DAPI nuclear stain (blue).
IHC testing of FFPE human cancer tissue with Presenilin 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HeLa cells with Presenilin 1 antibody; Blue=isotype control, Green= Presenilin 1 antibody.

Documents

Further Information

Application Details :
Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE): 1:50-1:100, Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Presenilin 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2) or the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma-secretase activity or themselves are protease enzymes.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 330-359 from the human protein was used as the immunogen for the Presenilin 1 antibody.
Limitation:
This Presenilin 1 antibody is available for research use only.
Localization:
Cytoplasmic, cell membrane
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
P49768