Dystrobrevin alpha Antibody / DTNA

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NSJ Bioreagents
Product Code: NSJ-F54653
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54653-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54653-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Dystrobrevin alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human 1) U-87 MG and 2) C2C12 cell lysate with Dystrobrevin alpha antibody. Predicted molecular weight ~84 kDa.
2 / 4
Western blot testing of human HepG2 cell lysate with Dystrobrevin alpha antibody. Predicted molecular weight ~84 kDa.
3 / 4
IHC testing of FFPE human brain tissue with Dystrobrevin alpha antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Immunofluorescent staining of human HEK293 cells with Dystrobrevin alpha antibody (green) and DAPI nuclear stain (blue).
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Western blot testing of human 1) U-87 MG and 2) C2C12 cell lysate with Dystrobrevin alpha antibody. Predicted molecular weight ~84 kDa.
Western blot testing of human HepG2 cell lysate with Dystrobrevin alpha antibody. Predicted molecular weight ~84 kDa.
IHC testing of FFPE human brain tissue with Dystrobrevin alpha antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HEK293 cells with Dystrobrevin alpha antibody (green) and DAPI nuclear stain (blue).

Documents

Further Information

Application Details :
Immunofluorescence: 1:25, Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Dystrobrevin alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene belongs to the dystrobrevin subfamily of the dystrophin family. This protein is a component of the dystrophin-associated protein complex (DPC), which consists of dystrophin and several integral and peripheral membrane proteins, including dystroglycans, sarcoglycans, syntrophins and alpha- and beta-dystrobrevin. The DPC localizes to the sarcolemma and its disruption is associated with various forms of muscular dystrophy. Mutations in this gene are associated with left ventricular noncompaction with congenital heart defects. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 692-721 from the human protein was used as the immunogen for the Dystrobrevin alpha antibody.
Limitation:
This Dystrobrevin alpha antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q9Y4J8