Paraoxonase 2 Antibody / PON2

NSJ Bioreagents
Product Code: NSJ-F54835
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54835-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54835-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Paraoxonase 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human placental tissue with Paraoxonase 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of human HepG2 cells with Paraoxonase 2 antibody (green) and DAPI nuclear stain (blue).
3 / 4
Western blot testing of human HepG2 cell lysate with Paraoxonase 2 antibody. Predicted molecular weight ~39 kDa.
4 / 4
Western blot testing of mouse liver tissue lysate with Paraoxonase 2 antibody. Predicted molecular weight ~39 kDa.

IHC testing of FFPE human placental tissue with Paraoxonase 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HepG2 cells with Paraoxonase 2 antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human HepG2 cell lysate with Paraoxonase 2 antibody. Predicted molecular weight ~39 kDa.
Western blot testing of mouse liver tissue lysate with Paraoxonase 2 antibody. Predicted molecular weight ~39 kDa.

Further Information

Application Details :
Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Paraoxonase 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PON2 is a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 74-101 from the human protein was used as the immunogen for the Paraoxonase 2 antibody.
Limitation:
This Paraoxonase 2 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q15165