TENT2 Antibody / GLD2

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NSJ Bioreagents
Product Code: NSJ-F54414
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54414-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54414-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the TENT2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human cerebellum tissue with TENT2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of human MCF7 cells with TENT2 antibody (green) and anti-Actin (red).
3 / 4
Western blot testing of 1) human HepG2 and 2) mouse brain lysate with TENT2 antibody. Predicted molecular weight ~56 kDa.
4 / 4
Western blot testing of mouse 1) brain and 2) cerebellulm lysate with TENT2 antibody. Predicted molecular weight ~56 kDa.
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IHC testing of FFPE human cerebellum tissue with TENT2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human MCF7 cells with TENT2 antibody (green) and anti-Actin (red).
Western blot testing of 1) human HepG2 and 2) mouse brain lysate with TENT2 antibody. Predicted molecular weight ~56 kDa.
Western blot testing of mouse 1) brain and 2) cerebellulm lysate with TENT2 antibody. Predicted molecular weight ~56 kDa.

Documents

Further Information

Application Details :
Immunofluorescence: 1:25,Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the TENT2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
GLD2 is cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. GLD2 does not play a role in replication-dependent histone mRNA degradation.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 58-87 from the human protein was used as the immunogen for the TENT2 antibody.
Limitation:
This TENT2 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q6PIY7