Cyclin T1 Antibody / CCNT1

Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells)

The stated application concentrations are suggested starting points. Titration of the CCNT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

CCNT1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin tightly associates with CDK9 kinase, and was found to be a major subunit of the transcription elongation factor p-TEFb. The kinase complex containing this cyclin and the elongation factor can interact with, and act as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and was shown to be both necessary and sufficient for full activation of viral transcription. This cyclin and its kinase partner were also found to be involved in the phosphorylation and regulation of the carboxy-terminal domain (CTD) of the largest RNA polymerase II subunit.

Purified

In 1X PBS, pH 7.4, with 0.09% sodium azide

A portion of amino acids 253-281 from the human protein was used as the immunogen for the CCNT1 antibody.

This CCNT1 antibody is available for research use only.

Antigen affinity purified

Human, Mouse

O60563