BCOR Antibody

NSJ Bioreagents
Product Code: NSJ-F54442
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54442-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54442-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the BCOR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
IHC testing of FFPE human breast cancer tissue with BCOR antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 5
IHC testing of FFPE mouse brain tissue with BCOR antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 5
Western blot testing of mouse liver lysate with BCOR antibody. Predicted molecular weight ~192 kDa.
4 / 5
Western blot testing of human HEK293 cell lysate with BCOR antibody. Predicted molecular weight ~192 kDa.
5 / 5
Flow cytometry testing of human HEK293 cells with BCOR antibody; Blue=isotype control, Green= BCOR antibody.

IHC testing of FFPE human breast cancer tissue with BCOR antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE mouse brain tissue with BCOR antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of mouse liver lysate with BCOR antibody. Predicted molecular weight ~192 kDa.
Western blot testing of human HEK293 cell lysate with BCOR antibody. Predicted molecular weight ~192 kDa.
Flow cytometry testing of human HEK293 cells with BCOR antibody; Blue=isotype control, Green= BCOR antibody.

Further Information

Application Details :
Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the BCOR antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
BCOR was identified as an interacting corepressor of BCL6, a POZ/zinc finger transcription repressor that is required for germinal center formation and may influence apoptosis. This protein selectively interacts with the POZ domain of BCL6, but not with eight other POZ proteins. Specific class I and II histone deacetylases (HDACs) have been shown to interact with this protein, which suggests a possible link between the two classes of HDACs.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1107-1137 from the human protein was used as the immunogen for the BCOR antibody.
Limitation:
This BCOR antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q6W2J9