CCR4-NOT transcription complex subunit 4 Antibody / CNOT4

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NSJ Bioreagents
Product Code: NSJ-F54694
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54694-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54694-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the CCR4-NOT transcription complex subunit 4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human 1) MCF7, 2) K562 and 3) HEK293 cell lysate with CCR4-NOT transcription complex subunit 4 antibody. Expected molecular weight: 48-84 kDa (multiple isoforms).
2 / 4
Western blot testing of mouse cerebellum tissue lysate with CCR4-NOT transcription complex subunit 4 antibody. Expected molecular weight: 48-84 kDa (multiple isoforms).
3 / 4
IHC testing of FFPE human testis tissue with CCR4-NOT transcription complex subunit 4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of fixed and permeabilized human U-2 OS cells with CCR4-NOT transcription complex subunit 4 antibody; Blue=isotype control, Green= CCR4-NOT transcription complex subunit 4 antibody.
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Western blot testing of human 1) MCF7, 2) K562 and 3) HEK293 cell lysate with CCR4-NOT transcription complex subunit 4 antibody. Expected molecular weight: 48-84 kDa (multiple isoforms).
Western blot testing of mouse cerebellum tissue lysate with CCR4-NOT transcription complex subunit 4 antibody. Expected molecular weight: 48-84 kDa (multiple isoforms).
IHC testing of FFPE human testis tissue with CCR4-NOT transcription complex subunit 4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of fixed and permeabilized human U-2 OS cells with CCR4-NOT transcription complex subunit 4 antibody; Blue=isotype control, Green= CCR4-NOT transcription complex subunit 4 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the CCR4-NOT transcription complex subunit 4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CNOT4 has intrinsic E3 ubiquitin ligase activity. Binding of the CNOT4 RING finger to the ubiquitin-conjugating enzyme (E2) UbcH5B is highly selective. The CCR4-NOT complex functions as a global regulator of RNA polymerase II transcription.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the CCR4-NOT transcription complex subunit 4 antibody.
Limitation:
This CCR4-NOT transcription complex subunit 4 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
O95628