Equilibrative nucleoside transporter 1 Antibody / ENT1

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NSJ Bioreagents
Product Code: NSJ-F54342
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54342-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54342-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the ENT1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of human 1) brain, 2) breast and 3) placenta lysate with ENT1 antibody. Predicted molecular weight ~50 kDa.
2 / 5
Western blot testing of human 1) heart, 2) breast and 3) placenta lysate with ENT1 antibody. Predicted molecular weight ~50 kDa.
3 / 5
Western blot testing of mouse 1) heart and 2) liver lysate with ENT1 antibody. Predicted molecular weight ~50 kDa.
4 / 5
IHC testing of FFPE human brain tissue with ENT1 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
5 / 5
Flow cytometry testing of fixed and permeabilized human HepG2 cells with ENT1 antibody; Blue=isotype control, Green= ENT1 antibody.
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Western blot testing of human 1) brain, 2) breast and 3) placenta lysate with ENT1 antibody. Predicted molecular weight ~50 kDa.
Western blot testing of human 1) heart, 2) breast and 3) placenta lysate with ENT1 antibody. Predicted molecular weight ~50 kDa.
Western blot testing of mouse 1) heart and 2) liver lysate with ENT1 antibody. Predicted molecular weight ~50 kDa.
IHC testing of FFPE human brain tissue with ENT1 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
Flow cytometry testing of fixed and permeabilized human HepG2 cells with ENT1 antibody; Blue=isotype control, Green= ENT1 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the ENT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ENT1, or Equilibrative nucleoside transporter 1, is a member of the equilibrative nucleoside transporter family. It is a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 402-431 from the human protein was used as the immunogen for the ENT1 antibody.
Limitation:
This ENT1 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q99808