HOMER3 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54437
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54437-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54437-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the HOMER3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
IHC testing of FFPE human brain tissue with HOMER3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 5
Western blot testing of human MDA-MB-231 cell lysate with HOMER3 antibody. Predicted molecular weight ~40 kDa, can be observed at 45-47 kDa.
3 / 5
Western blot testing of mouse NIH 3T3 cell lysate with HOMER3 antibody. Predicted molecular weight ~40 kDa, can be observed at 45-47 kDa.
4 / 5
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with HOMER3 antibody.
5 / 5
Flow cytometry testing of human SK-BR-3 cells with HOMER3 antibody; Blue=isotype control, Green= HOMER3 antibody.
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IHC testing of FFPE human brain tissue with HOMER3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human MDA-MB-231 cell lysate with HOMER3 antibody. Predicted molecular weight ~40 kDa, can be observed at 45-47 kDa.
Western blot testing of mouse NIH 3T3 cell lysate with HOMER3 antibody. Predicted molecular weight ~40 kDa, can be observed at 45-47 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with HOMER3 antibody.
Flow cytometry testing of human SK-BR-3 cells with HOMER3 antibody; Blue=isotype control, Green= HOMER3 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the HOMER3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
HOMER3 is a member of the HOMER family of postsynaptic density scaffolding proteins that share a similar domain structure consisting of an N-terminal Enabled/vasodilator-stimulated phosphoprotein homology 1 domain which mediates protein-protein interactions, and a carboxy-terminal coiled-coil domain and two leucine zipper motifs that are involved in self-oligomerization. This protein binds numerous other proteins including group I metabotropic glutamate receptors,inositol 1,4,5-trisphosphate receptors and amyloid precursor proteins and has been implicated in diverse biological functions such as neuronal signaling, T-cell activation and trafficking of amyloid beta peptides.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 281-308 from the human protein was used as the immunogen for the HOMER3 antibody.
Limitation:
This HOMER3 antibody is available for research use only.
Localization:
Cytoplasmic, cell junction
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q9NSC5