Pro-MCH Antibody / PMCH

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NSJ Bioreagents
Product Code: NSJ-F54416
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54416-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54416-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Pro-MCH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of mouse brain lysate with Pro-MCH antibody.
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Western blot testing of human K562 cell lysate with Pro-MCH antibody.
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Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Pro-MCH antibody.
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IHC testing of FFPE human testis tissue with Pro-MCH antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Flow cytometry testing of human K562 cells with Pro-MCH antibody; Blue=isotype control, Green= Pro-MCH antibody.
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Western blot testing of mouse brain lysate with Pro-MCH antibody.
Western blot testing of human K562 cell lysate with Pro-MCH antibody.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Pro-MCH antibody.
IHC testing of FFPE human testis tissue with Pro-MCH antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human K562 cells with Pro-MCH antibody; Blue=isotype control, Green= Pro-MCH antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the Pro-MCH antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The melanin-concentrating hormone (MCH) is a cyclic neuropeptide isolated initially from salmon pituitary gland and later from rat hypothalamus (summarized by Nahon et al., 1992 [PubMed 1572663]). In mammals, MCH perikarya are confined largely to the lateral hypothalamus and zona incerta area with extensive neuronal projections throughout the brain, including the neurohypophysis. The anatomic distribution suggests a neurotransmitter or neuromodulator role for MCH in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal. MCH and 2 other putative neuropeptides, NEI and NGE, are encoded by the same precursor and appear colocalized in nerve cells and in many instances within the projections. The precursor is designated pro-melanin-concentrating hormone (PMCH).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 94-122 from the human protein was used as the immunogen for the Pro-MCH antibody.
Limitation:
This Pro-MCH antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P20382