TCF3 Antibody / Transcription factor E2-alpha

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NSJ Bioreagents
Product Code: NSJ-F54373
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54373-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54373-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the TCF3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human tonsil tissue with TCF3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of human 1) Jurkat and 2) Ramos cell lysate with TCF3 antibody. Expected molecular weight: 68-78 kDa.
3 / 4
Western blot testing of mouse cerebellum tissue lysate with TCF3 antibody. Expected molecular weight: 68-78 kDa.
4 / 4
Flow cytometry testing of human A549 cells with TCF3 antibody; Blue=isotype control, Green= TCF3 antibody.
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IHC testing of FFPE human tonsil tissue with TCF3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) Jurkat and 2) Ramos cell lysate with TCF3 antibody. Expected molecular weight: 68-78 kDa.
Western blot testing of mouse cerebellum tissue lysate with TCF3 antibody. Expected molecular weight: 68-78 kDa.
Flow cytometry testing of human A549 cells with TCF3 antibody; Blue=isotype control, Green= TCF3 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the TCF3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The TCF3 gene, also called E2A, encodes 2 basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. E12 and E47 are involved in regulation of immunoglobulin gene expression.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 506-534 from the human protein was used as the immunogen for the TCF3 antibody.
Limitation:
This TCF3 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P15923