Vinculin Antibody

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NSJ Bioreagents
Product Code: NSJ-F54324
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54324-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54324-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Vinculin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE mouse skeletal muscle tissue with Vinculin antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of 1) human skeletal muscle, 2) human A431, 3) human HeLa, 4) mouse kidney, 5) rat PC-12 and 6) human HepG2 lysate with Vinculin antibody. Predicted molecular weight ~124 kDa.
3 / 4
Flow cytometry testing of fixed and permeabilized mouse NIH 3T3 cells with Vinculin antibody; Blue=isotype control, Green= Vinculin antibody.
4 / 4
Flow cytometry testing of fixed and permeabilized mouse C2C12 cells with Vinculin antibody; Blue=isotype control, Green= Vinculin antibody.
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IHC testing of FFPE mouse skeletal muscle tissue with Vinculin antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of 1) human skeletal muscle, 2) human A431, 3) human HeLa, 4) mouse kidney, 5) rat PC-12 and 6) human HepG2 lysate with Vinculin antibody. Predicted molecular weight ~124 kDa.
Flow cytometry testing of fixed and permeabilized mouse NIH 3T3 cells with Vinculin antibody; Blue=isotype control, Green= Vinculin antibody.
Flow cytometry testing of fixed and permeabilized mouse C2C12 cells with Vinculin antibody; Blue=isotype control, Green= Vinculin antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the Vinculin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 903-937 from the human protein was used as the immunogen for the Vinculin antibody.
Limitation:
This Vinculin antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q64727