14-3-3 gamma Antibody / YWHAG

NSJ Bioreagents
Product Code: NSJ-F54406
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54406-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54406-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the YWHAG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
IHC testing of FFPE human colon carcinoma tissue with YWHAG antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 5
Western blot testing of human HeLa cell lysate with YWHAG antibody. Predicted molecular weight ~28 kDa.
3 / 5
Western blot testing of mouse cerebellum lysate with YWHAG antibody. Predicted molecular weight ~28 kDa.
4 / 5
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with YWHAG antibody.
5 / 5
Flow cytometry testing of human WiDr cells with YWHAG antibody; Blue=isotype control, Green= YWHAG antibody.

IHC testing of FFPE human colon carcinoma tissue with YWHAG antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human HeLa cell lysate with YWHAG antibody. Predicted molecular weight ~28 kDa.
Western blot testing of mouse cerebellum lysate with YWHAG antibody. Predicted molecular weight ~28 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with YWHAG antibody.
Flow cytometry testing of human WiDr cells with YWHAG antibody; Blue=isotype control, Green= YWHAG antibody.

Further Information

Application Details :
Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the YWHAG antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
YWHAG belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 136-164 from the human protein was used as the immunogen for the YWHAG antibody.
Limitation:
This YWHAG antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
SAS precipitation
Species Reactivity :
Human, Mouse
Uniprot #:
P61981