ZWINT Antibody

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NSJ Bioreagents
Product Code: NSJ-F54426
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54426-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54426-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the ZWINT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of mouse cerebellum lysate with ZWINT antibody. Predicted molecular weight ~31 kDa.
2 / 5
Western blot testing of human MOLT4 lysate with ZWINT antibody. Predicted molecular weight ~31 kDa.
3 / 5
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with ZWINT antibody.
4 / 5
IHC testing of FFPE human brain tissue with ZWINT antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 5
Flow cytometry testing of human WiDr cells with ZWINT antibody; Blue=isotype control, Green= ZWINT antibody.
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Western blot testing of mouse cerebellum lysate with ZWINT antibody. Predicted molecular weight ~31 kDa.
Western blot testing of human MOLT4 lysate with ZWINT antibody. Predicted molecular weight ~31 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with ZWINT antibody.
IHC testing of FFPE human brain tissue with ZWINT antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human WiDr cells with ZWINT antibody; Blue=isotype control, Green= ZWINT antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the ZWINT antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ZWINT is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 59-88 from the human protein was used as the immunogen for the ZWINT antibody.
Limitation:
This ZWINT antibody is available for research use only.
Purity:
SAS precipitation
Species Reactivity :
Human, Mouse
Uniprot #:
O95229