Inhibin alpha Antibody / INHA

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NSJ Bioreagents
Product Code: NSJ-F54878
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54878-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54878-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Inhibin alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Flow cytometry testing of human HEK293 cells with Inhibin alpha antibody; Blue=isotype control, Green= Inhibin alpha antibody.
2 / 4
IHC testing of FFPE human testis tissue with Inhibin alpha antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of human HeLa cells with Inhibin alpha antibody (green) and DAPI nuclear stain (blue).
4 / 4
Western blot testing of 1) human SK-BR-3 and 2) mouse ovary lysate with Inhibin alpha antibody. Predicted molecular weight ~40 kDa.
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Flow cytometry testing of human HEK293 cells with Inhibin alpha antibody; Blue=isotype control, Green= Inhibin alpha antibody.
IHC testing of FFPE human testis tissue with Inhibin alpha antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HeLa cells with Inhibin alpha antibody (green) and DAPI nuclear stain (blue).
Western blot testing of 1) human SK-BR-3 and 2) mouse ovary lysate with Inhibin alpha antibody. Predicted molecular weight ~40 kDa.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:10-1:50, Immunofluorescence: 1:10-1:50
Application Note:
The stated application concentrations are suggested starting points. Titration of the Inhibin alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The inhibin alpha subunit joins either the beta A or beta B subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumour-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. However, in prostate cancer, expression of the inhibin alpha-subunit gene was suppressed and was not detectable in poorly differentiated tumor cells. Furthermore, because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 79-108 from the human protein was used as the immunogen for the Inhibin alpha antibody.
Limitation:
This Inhibin alpha antibody is available for research use only.
Localization:
Nuclear and Cytoplasmic (secreted)
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P05111