RARA Antibody / Retinoic Acid Receptor Alpha

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NSJ Bioreagents
Product Code: NSJ-F54372
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54372-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54372-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the RAR alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
IHC testing of FFPE mouse cerebellum tissue with RAR alpha antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 5
Immunofluorescent staining of fixed and permeabilized human MCF7 cells with Cytokeratin 18 antibody (green) and DAPI nuclear stain (blue).
3 / 5
Western blot testing of 1) human HL60 and 2) mouse testis lysate with RAR alpha antibody. Predicted molecular weight ~51 kDa.
4 / 5
Western blot testing of human 1) HL60, 2) MCF7, 3) HepG2, 4) HeLa and 5) T-47D cell lysate with RAR alpha antibody. Predicted molecular weight ~51 kDa.
5 / 5
Flow cytometry testing of human HeLa cells with RAR alpha antibody; Blue=isotype control, Green= RAR alpha antibody.
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IHC testing of FFPE mouse cerebellum tissue with RAR alpha antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human MCF7 cells with Cytokeratin 18 antibody (green) and DAPI nuclear stain (blue).
Western blot testing of 1) human HL60 and 2) mouse testis lysate with RAR alpha antibody. Predicted molecular weight ~51 kDa.
Western blot testing of human 1) HL60, 2) MCF7, 3) HepG2, 4) HeLa and 5) T-47D cell lysate with RAR alpha antibody. Predicted molecular weight ~51 kDa.
Flow cytometry testing of human HeLa cells with RAR alpha antibody; Blue=isotype control, Green= RAR alpha antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the RAR alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 322-349 from the human protein were used as the immunogen for the RAR alpha antibody.
Limitation:
This RAR alpha antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P10276