CBFB Antibody

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NSJ Bioreagents
Product Code: NSJ-F54434
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54434-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54434-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the CBFB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of 1) human K562 and 2) mouse lung lysate with CBFB antibody. Predicted molecular weight ~22 kDa.
2 / 5
Western blot testing of human 1) Jukat and 2) Raji cell lysate with CBFB antibody. Predicted molecular weight ~22 kDa.
3 / 5
Immunofluorescent staining of human HEK293 cells with CBFB antibody (green) and DAPI nuclear stain (blue).
4 / 5
IHC testing of FFPE human brain tissue with CBFB antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 5
Flow cytometry testing of human HL60 cells with CBFB antibody; Blue=isotype control, Green= CBFB antibody.
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Western blot testing of 1) human K562 and 2) mouse lung lysate with CBFB antibody. Predicted molecular weight ~22 kDa.
Western blot testing of human 1) Jukat and 2) Raji cell lysate with CBFB antibody. Predicted molecular weight ~22 kDa.
Immunofluorescent staining of human HEK293 cells with CBFB antibody (green) and DAPI nuclear stain (blue).
IHC testing of FFPE human brain tissue with CBFB antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HL60 cells with CBFB antibody; Blue=isotype control, Green= CBFB antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the CBFB antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Core-binding factor subunit beta is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 61-90 from the human protein was used as the immunogen for the CBFB antibody.
Limitation:
This CBFB antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q13951