Antithrombin III Antibody

NSJ Bioreagents
Product Code: NSJ-F54427
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54427-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54427-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Serpin C1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of human blood plasma lysate with Serpin C1 antibody. Predicted molecular weight ~52 kDa.
2 / 5
Western blot testing of mouse liver lysate with Serpin C1 antibody. Predicted molecular weight ~52 kDa.
3 / 5
Immunofluorescent staining of human HEK293 cells with Serpin C1 antibody (green) and DAPI nuclear stain (blue).
4 / 5
IHC testing of FFPE human hepatocarcinoma tissue with Serpin C1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 5
Flow cytometry testing of human HepG2 cells with Serpin C1 antibody; Blue=isotype control, Green= Serpin C1 antibody.

Western blot testing of human blood plasma lysate with Serpin C1 antibody. Predicted molecular weight ~52 kDa.
Western blot testing of mouse liver lysate with Serpin C1 antibody. Predicted molecular weight ~52 kDa.
Immunofluorescent staining of human HEK293 cells with Serpin C1 antibody (green) and DAPI nuclear stain (blue).
IHC testing of FFPE human hepatocarcinoma tissue with Serpin C1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HepG2 cells with Serpin C1 antibody; Blue=isotype control, Green= Serpin C1 antibody.

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the Serpin C1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
SERPINC1 is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. The protein includes two functional domains: the heparin binding-domain at the N-terminus of the mature protein, and the reactive site domain at the C-terminus. The inhibitory activity is enhanced by the presence of heparin.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 364-393 from the human protein was used as the immunogen for the Serpin C1 antibody.
Limitation:
This Serpin C1 antibody is available for research use only.
Purity:
SAS precipitation
Species Reactivity :
Human, Mouse
Uniprot #:
P01008