AP1M1 Antibody

NSJ Bioreagents
Product Code: NSJ-F54275
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54275-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54275-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the AP1M1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of 1) human PC-3, 2) human U-251 MG, 3) mouse NIH 3T3 and 4) mouse heart lysate with AP1M1 antibody. Predicted molecular weight ~49 kDa.
2 / 4
Western blot testing of human brain lysate with AP1M1 antibody. Predicted molecular weight ~49 kDa.
3 / 4
IHC testing of FFPE human brain tissue with AP1M1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Immunofluorescent staining of fixed and permeabilized human A375 cells with AP1M1 antibody (green) and DAPI nuclear stain (blue).

Western blot testing of 1) human PC-3, 2) human U-251 MG, 3) mouse NIH 3T3 and 4) mouse heart lysate with AP1M1 antibody. Predicted molecular weight ~49 kDa.
Western blot testing of human brain lysate with AP1M1 antibody. Predicted molecular weight ~49 kDa.
IHC testing of FFPE human brain tissue with AP1M1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human A375 cells with AP1M1 antibody (green) and DAPI nuclear stain (blue).

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunofluorescence: 1:25,Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the AP1M1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene is the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The other components of this complex are beta-prime-adaptin, gamma-adaptin, and the small chain AP1S1. This complex is located at the Golgi vesicle and links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. [provided by RefSeq].
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 199-227 from the human protein were used as the immunogen for the AP1M1 antibody.
Limitation:
This AP1M1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q9BXS5