p21 Antibody / CDKN1A

Western blot: 1:500-1:2000, Immunofluorescence: 1:25, Immunohistochemistry (FFPE): 1:25

The stated application concentrations are suggested starting points. Titration of the CDKN1A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene.

Purified

In 1X PBS, pH 7.4, with 0.09% sodium azide

A portion of amino acids 117-146 from the human protein was used as the immunogen for the CDKN1A antibody.

This CDKN1A antibody is available for research use only.

Nuclear, cytoplasmic

Antigen affinity purified

Human, Mouse

P38936