HTRA1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54502
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54502-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54502-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the HTRA1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Immunofluorescent staining of human HeLa cells with HTRA1 antibody (green) and DAPI nuclear stain (blue).
2 / 4
Western blot testing of human HeLa cell lysate with HTRA1 antibody. Predicted molecular weight ~51 kDa.
3 / 4
Western blot testing of mouse brain lysate with HTRA1 antibody. Predicted molecular weight ~51 kDa.
4 / 4
IHC testing of FFPE human kidney tissue with HTRA1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Immunofluorescent staining of human HeLa cells with HTRA1 antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human HeLa cell lysate with HTRA1 antibody. Predicted molecular weight ~51 kDa.
Western blot testing of mouse brain lysate with HTRA1 antibody. Predicted molecular weight ~51 kDa.
IHC testing of FFPE human kidney tissue with HTRA1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Immunofluorescence: 1:25, Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the HtrA1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
HtrA1 is a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 116-147 from the human protein was used as the immunogen for the HTRA1 antibody.
Limitation:
This HTRA1 antibody is available for research use only.
Localization:
Cytoplasmic, cell membrane
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q92743