Peroxiredoxin 2 Antibody / PRDX2

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NSJ Bioreagents
Product Code: NSJ-F54797
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54797-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54797-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the PRDX2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of 1) human MDA-MB-231, 2) HEK293 and 3) mouse brain lysate with PRDX2 antibody. Predicted molecular weight ~22 kDa.
2 / 4
Western blot testing of 1) mouse heart and 2) rat heart tissue lysate with PRDX2 antibody. Predicted molecular weight ~22 kDa.
3 / 4
Immunofluorescent staining of fixed and permeabilized human HeLa cells with PRDX2 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 4
IHC testing of FFPE human brain tissue with PRDX2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of 1) human MDA-MB-231, 2) HEK293 and 3) mouse brain lysate with PRDX2 antibody. Predicted molecular weight ~22 kDa.
Western blot testing of 1) mouse heart and 2) rat heart tissue lysate with PRDX2 antibody. Predicted molecular weight ~22 kDa.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with PRDX2 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
IHC testing of FFPE human brain tissue with PRDX2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE): 1:50-1:100
Application Note:
The stated application concentrations are suggested starting points. Titration of the PRDX2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PRDX2 is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. This protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. The crystal structure of this protein has been resolved to 2.7 angstroms.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 169-198 from the human protein was used as the immunogen for the PRDX2 antibody.
Limitation:
This PRDX2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
P32119