ENO1 Antibody / Enolase 1 / Alpha Enolase

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NSJ Bioreagents
Product Code: NSJ-F54858
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54858-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54858-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the ENO1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of 1) human Jurkat, 2) human MCF7, 3) human U-251, 4) mouse NIH 3T3 and 5) mouse brain lysate with ENO1 antibody. Predicted molecular weight ~47 kDa.
2 / 4
IHC testing of FFPE human lymph tissue with ENO1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of fixed and permeabilized mouse C2C12 cells with ENO1 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 4
Flow cytometry testing of human HeLa cells with ENO1 antibody; Left=isotype control, Right= ENO1 antibody.
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Western blot testing of 1) human Jurkat, 2) human MCF7, 3) human U-251, 4) mouse NIH 3T3 and 5) mouse brain lysate with ENO1 antibody. Predicted molecular weight ~47 kDa.
IHC testing of FFPE human lymph tissue with ENO1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized mouse C2C12 cells with ENO1 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Flow cytometry testing of human HeLa cells with ENO1 antibody; Left=isotype control, Right= ENO1 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE): 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the ENO1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ENO1 is one of three enolase isoenzymes found in mammals; it is alpha-enolase, a homodimeric soluble enzyme, and also a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 178-205 from the human protein was used as the immunogen for the ENO1 antibody.
Limitation:
This ENO1 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
P06733