KLF6 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54861
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54861-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54861-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the KLF6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of mouse stomach lysate with KLF6 antibody. Expected molecular weight: 32-40 kDa.
2 / 4
Immunofluorescent staining of fixed and permeabilized human HeLa cells with KLF6 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
3 / 4
IHC testing of FFPE human testis tissue with KLF6 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human WiDr cells with KLF6 antibody; Blue=isotype control, Green= KLF6 antibody.
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Western blot testing of mouse stomach lysate with KLF6 antibody. Expected molecular weight: 32-40 kDa.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with KLF6 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
IHC testing of FFPE human testis tissue with KLF6 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human WiDr cells with KLF6 antibody; Blue=isotype control, Green= KLF6 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE): 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the KLF6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
COPEB is a member of the Kruppel-like family of transcription factors. The zinc finger protein is a transcriptional activator, and functions as a tumor suppressor.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 159-186 from the human protein was used as the immunogen for the KLF6 antibody.
Limitation:
This KLF6 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q99612