Annexin A2 Antibody / ANXA2

NSJ Bioreagents
Product Code: NSJ-F54307
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54307-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54307-0.4ML0.4 ml£469.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Annexin A2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human liver tissue with Annexin A2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
IHC testing of FFPE human lung carcinoma tissue with Annexin A2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Western blot testing of 1) human HeLa, 2) human MCF7, 3) mouse NIH 3T3, 4) human SH-SY5Y and 5) human SK-BR-3 cell lysate with Annexin A2. Predicted molecular weight ~38 kDa.
4 / 4
Western blot testing of mouse lung lysate with Annexin A2. Predicted molecular weight ~38 kDa.

IHC testing of FFPE human liver tissue with Annexin A2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human lung carcinoma tissue with Annexin A2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of 1) human HeLa, 2) human MCF7, 3) mouse NIH 3T3, 4) human SH-SY5Y and 5) human SK-BR-3 cell lysate with Annexin A2. Predicted molecular weight ~38 kDa.
Western blot testing of mouse lung lysate with Annexin A2. Predicted molecular weight ~38 kDa.

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the Annexin A2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ANXA2 is a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 19-49 from the human protein were used as the immunogen for the Annexin A2 antibody.
Limitation:
This Annexin A2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P07355