Bcl2L2 Antibody / Bcl-W (BH3 domain specific)

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NSJ Bioreagents
Product Code: NSJ-F54369
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54369-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54369-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Bcl-W antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human breast cancer tissue with Bcl-W antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 3
Western blot testing of human HL60 cell lysate with Bcl-W antibody. Predicted moleuclar weight ~21 kDa.
3 / 3
Western blot testing of mouse NIH 3T3 cell lysate with Bcl-W antibody. Predicted moleuclar weight ~21 kDa.
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IHC testing of FFPE human breast cancer tissue with Bcl-W antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human HL60 cell lysate with Bcl-W antibody. Predicted moleuclar weight ~21 kDa.
Western blot testing of mouse NIH 3T3 cell lysate with Bcl-W antibody. Predicted moleuclar weight ~21 kDa.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the Bcl-W antibody (BH3 Domain Specific) may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Bcl-w is a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 24-59 from the human protein was used as the immunogen for the Bcl-W antibody.
Limitation:
This Bcl-W antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
SAS precipitation
Species Reactivity :
Human, Mouse
Uniprot #:
Q92843