CTSA Antibody / Cathepsin A

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NSJ Bioreagents
Product Code: NSJ-F54269
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54269-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54269-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Cathepsin A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
IHC testing of FFPE human testis tissue with Cathepsin A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 5
IHC testing of FFPE human kidney tissue with Cathepsin A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 5
Western blot testing of human 1) A431, 2) HeLa, 3) HepG2, 4) HT-1080, 5) placenta and 6) kidney lysate with Cathepsin A antibody. Expected molecular weight: ~54 kDa precursor, 32 kDa (large subunit).
4 / 5
Western blot testing of human HEK293 cell lysate with Cathepsin A antibody. Expected molecular weight: ~54 kDa precursor, 32 kDa (large subunit).
5 / 5
Western blot testing of mouse bladder lysate with Cathepsin A antibody. Expected molecular weight: ~54 kDa precursor, 32 kDa (large subunit).
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IHC testing of FFPE human testis tissue with Cathepsin A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human kidney tissue with Cathepsin A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) A431, 2) HeLa, 3) HepG2, 4) HT-1080, 5) placenta and 6) kidney lysate with Cathepsin A antibody. Expected molecular weight: ~54 kDa precursor, 32 kDa (large subunit).
Western blot testing of human HEK293 cell lysate with Cathepsin A antibody. Expected molecular weight: ~54 kDa precursor, 32 kDa (large subunit).
Western blot testing of mouse bladder lysate with Cathepsin A antibody. Expected molecular weight: ~54 kDa precursor, 32 kDa (large subunit).

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the Cathepsin A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CTSA encodes a glycoprotein which associates with lysosomal enzymes beta-galactosidase and neuraminidase to form a complex of high molecular weight multimers. The formation of this complex provides a protective role for stability and activity. Deficiencies in this gene are linked to multiple forms of galactosialidosis.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 18-45 from the human protein were used as the immunogen for the Cathepsin A antibody.
Limitation:
This Cathepsin A antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P10619