GPX3 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54583
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54583-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54583-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the GPX3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of 1) human kidney, 2) human plasma, 3) mouse kidney, 4) mouse plasma and 5) rat kidney lysate with GPX3 antibody. Expected molecular weight: 23-26 kDa.
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Western blot testing of mouse plasma lysate with GPX3 antibody. Expected molecular weight: 23-26 kDa.
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IHC testing of FFPE human kidney tissue with GPX3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of 1) human kidney, 2) human plasma, 3) mouse kidney, 4) mouse plasma and 5) rat kidney lysate with GPX3 antibody. Expected molecular weight: 23-26 kDa.
Western blot testing of mouse plasma lysate with GPX3 antibody. Expected molecular weight: 23-26 kDa.
IHC testing of FFPE human kidney tissue with GPX3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the GPX3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 93-123 from the human protein was used as the immunogen for the GPX3 antibody.
Limitation:
This GPX3 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P22352