IDE Antibody / Insulin degrading enzyme

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NSJ Bioreagents
Product Code: NSJ-F54376
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54376-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54376-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the IDE antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human hepatocellular carcinoma tissue with IDE antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.~
2 / 2
Western blot testing of human 1) HeLa, 2) HepG2, 3) A375, 4) K562 a
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IHC testing of FFPE human hepatocellular carcinoma tissue with IDE antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.~
Western blot testing of human 1) HeLa, 2) HepG2, 3) A375, 4) K562 a

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the IDE antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
IDE belongs to a protease family responsible for intercellular peptide signalling. Though its role in the cellular processing of insulin has not yet been defined, insulin-degrading enzyme is thought to be involved in the termination of the insulin response.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 406-435 from the human protein was used as the immunogen for the IDE antibody.
Limitation:
This IDE antibody is available for research use only.
Purity:
SAS precipitation
Species Reactivity :
Human, Mouse
Uniprot #:
P14735